// BIOZAR LLC · Krasnodar Krai, Russia · essential oils · BIOSTAT

Essential oils, and a crop-protection product made from one of them.

BIOZAR makes coriander, fennel, dill, chamomile and fir oil. BIOSTAT is its insecticide, acaricide and fungicide on coriander essential oil — patented, listed for nine crops in its registration dossier, and field-tested against the oak lace bug by drone.

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// Field trial · oak lace bug · Corythucha arcuata · North-West Caucasus, 2016–2020Invasion area may exceed 7 million ha — the report's estimate

98.5% in seven days: the canopy comes back.

Seven days after one drone pass of Biostat MK at 3.0 l/ha, the oak lace bug is down 98.5% against the untreated control and the leaf surface is recovering. BIOSTAT is an insecticide, acaricide and fungicide on coriander essential oil, in the registration dossier for nine crops from winter wheat to grape. Patent RU 2762422.

See the seven-day trial BIOSTAT specification

Biostat MK · DJI Agras T-16 · ultra-low-volume (ULV) sprayer · 3.0 l/ha. Reduction of adults and larvae against the untreated control, oak stands, third ten-day period of August.

Day 126.6%after one pass
Day 390.8%adults and larvae down
Day 798.5%leaves recovering

Fig. 1 Spectrum and efficacy

93–99% on mites. 56–59% on sunn pest.
Thirteen rows, all on the page.

BIOSTAT's registration dossier lists nine crops — winter wheat, tomato, cucumber, watermelon, melon, tobacco, apple, peach and grape — and thirteen efficacy rows against aphids, mites, thrips, cotton bollworm, sunn pest, leaf curl, brown rust, septoria and root rots. The weakest row is set at the same size as the strongest.

Winter wheat
Tomato
Cucumber
Watermelon
Melon
Tobacco
Apple
Peach
Grape

Rates, schedule and the crop × target matrix

Crop / situation	Target	Efficacy
Vegetables, protected ground	aphids	72–98%
Vegetables, protected ground	thrips	70–80%
Vegetables, protected ground	mites	93–99%
Vegetables, open ground	aphids	88–92%
Tomato, open ground	cotton bollworm	70–88%
Melons and gourds	aphids	88–98%
Peach	aphids	88–95%
Peach	leaf curl	70–98%
Tobacco	aphids	83–85%
Tobacco	cotton bollworm	83–86%
Tobacco	thrips	87–92%
Wheat	root rots, brown rust, septoria	70–80%
Wheat	sunn pest	56–59%Kept on the page

Biological efficacy by crop and target, in the order and wording of the registration dossier; also reported in the product description and the company deck.

Fig. 2 BIOSTAT in one screen

220 g/l of coriander essential oil, in an emulsion concentrate.

Every figure below is from the registration dossier signed by the company's scientific supervisor. Where a study has not been done, the cell says so.

The full specification

Active substance220 ± 10g/l

Coriander essential oil, (+)-linalool ~65–70%, as an emulsion concentrate (EC) diluted in water for spraying.

Speed of action1hour

Effect from 1 hour after treatment. Systemic: works through the whole plant.

Protective period15days

10–25 depending on the pest, its density and generation speed.

Pre-harvest interval3days

5 days in greenhouses.

Rate by crop group0.3–1.5l/ha

Field crops 0.3–0.6; orchards and vineyards 0.75–1.5; greenhouses 0.3–0.75.

Hazard class, GOST 12.1.007Class 3

Moderately hazardous under GOST 12.1.007, the Russian state standard. Oral LD50 (median lethal dose) of the formulation 4,370–4,490 mg/kg.

Patent, 10 June 2021RU 2762422

Coriander essential oil and α-pinene, mass ratio 1:4 to 4:1.

Tank mix

Compatible with chemical insecticides, fungicides, herbicides and fertilisers; not with microbiological preparations.

Plants

Not phytotoxic at recommended doses; above them, flower burns are possible.

Flammable

Flash point 62 °C, ignition temperature 243 °C; fire- and explosion-hazardous.

Fig. 3 Eight variants, one control

88.9–98.5% on day 7.

Eight preparations — three Biostat compositions and five single oils — applied by backpack sprayer and by an unmanned aerial vehicle (UAV), third ten-day period of August. Pre-treatment counts 7–36 individuals per leaf; untreated control 15–30. Efficacy is the reduction in pest numbers against the control. Photosynthetic capacity was read by multispectral sensing before treatment and 7 and 14 days after.

Biostat M — coriander oil. Biostat MS — coriander, fennel and dill oils. Biostat MK — coriander oil with α-pinene and fennel oil; the proportions are not given in the report.

The trial in full, with the leaf strips

Pass 01 · Biostat MK · UAV, ULV · 3.0 l/ha

Variant	l/ha	Day 1	Day 3	Day 7
Biostat MKUAV, ULV	3.0	26.6	90.8	98.5
Coriander oilBackpack	2.0	36.1	70.6	96.7
Fir oilBackpack	2.0	70.2	87.4	88.9
Fennel oilBackpack	3.0	66.8	86.6	96.8
Dill oilBackpack	3.0	51.4	79.2	95.8
Chamomile oilBackpack	2.0	40.9	75.8	94.1
Biostat MBackpack	2.0	35.0	68.2	95.4
Biostat MSBackpack	2.0	76.6	76.6	94.1

Biological efficacy, %: death of adults and larvae against the untreated control. Biostat MK applied by a DJI Agras T-16 with a ULV sprayer; the seven others by a Pulverex backpack sprayer.

Method reduction vs untreated control Pre-treatment 7–36 per leaf Applied third ten-day period of August Sensing multispectral, day 0 / 7 / 14

Fig. 4 Application from the air

3.0 l/ha from a DJI Agras T-16.

Ultra-low-volume spraying of oaks standing in mixed forest, dosed to the tree. BIOSTAT is also applied with hand-held sprayers, orchard fan sprayers and field boom sprayers, and is compatible with standard spraying systems.

A spraying drone hovering above a stand of oaks at a field edge, beside a dirt track and a ploughed field, under a clear late-summer sky. — **Fig. 4** — photograph, company archive

Fig. 5 Composition and toxicology

(+)-Linalool, 65–70% of the oil.

(+)-linalool~65–70
(+)-α-pinene8–12
geraniol3–9
camphor3–6
γ-terpinene1.5–3
(−)-borneol1–3
8 more components →

Mass fractions of the coriander essential oil, the six largest of fourteen.

4,490 mg/kg rats · 4,370 mg/kg miceOral LD50 (median lethal dose), formulation, 5% aqueous solutionHazard class 3, GOST 12.1.007 (the Russian state standard)
> 2,500 mg/kgDermal LD50, formulationHighest dose tested
10%Eye mucosa — threshold concentration of the aqueous emulsion for local irritant action
62 °CFlash pointFlammable

Where the company's partner deck states a higher acute-toxicity figure, it is not used on this site; the registration dossier's figures are.

Toxicology in full

Fig. 6 Developed with

Developed with the Federal Research Centre of Biological Plant Protection, Krasnodar.

Laboratory of Chemical Communication and Mass Rearing of Insects

Field trials of the preparations, led by its head Vladimir Yakovlevich Ismailov — Candidate of Biological Sciences, leading researcher and the scientific supervisor of BIOZAR LLC, who signed the registration dossier.

Moscow Institute of Medical Parasitology, Tropical and Transmissible Diseases

Toxicology of the active substance and of the formulation.

Patent RU 2762422, 10 June 2021

“An insecto-acaricidal composition characterised in that it comprises coriander essential oil and α-pinene in a mass ratio from 1:4 to 4:1.” Covers the composition and its extraction.

The institute and the patent

Fig. 7 Essential oils

21,905 kg of essential oil exported in four months of 2021.

Coriander seed oil19,745kg

5 contracts.

Fennel oil2,160kg

1 contract.

TotalUSD 1,202,340

6 contracts, 2 buyers, 2 countries — the United Kingdom and Switzerland — August–November 2021.

Dried coriander seeds heaped close to the lens, round and ridged, in soft side light. — **Fig. 7** — photograph, CC BY-SA 4.0, Sanjay Acharya

Coriander — main product
Fennel
Dill
Chamomile
Fir

Directions, not capacity

Direction
Growing essential-oil crops on up to 500 ha of irrigated land — a preliminary agreement with a landowner — and distilling oil from fresh-cut herbage in the off-season.
Direction
Fennel and dill hydrolates, up to 200,000 l/year.
Direction
Coriander meal, dried and granulated — interest from beef and dairy cattle farms.
Direction
Spices for the food industry.
Direction
Bottled drinking water with fennel and dill hydrolates; groundwater licence for up to 500 m³ — period not stated in the source.

Development directions from the plant's own document. None is delivered capacity.

The export ledger and the oils

Fig. 8 The team

Four people, one laboratory, one plant.

Vladimir Yakovlevich IsmailovCandidate of Biological Sciences; scientific supervisor of BIOZAR LLC
Vladimir Evgenievich KrepakProject lead; general director of the essential-oil plant
Sergey Viktorovich KozyryalovFirst deputy project lead; commercial director of the essential-oil plant
Olga Nikolaevna SpodaChief production technologist